武汉艾必赛生物有限公司推荐Yersinia pestis F1 capsule antigen/Caf1 Recombinant Protein ,精品蛋白

链接：https://www.abinscience.cn/product/78/52546.html

名称：Yersinia pestis F1 capsule antigen/Caf1 Recombinant Protein

货号：JN173012

表达系统：E. coli

Accession号：P26948

蛋白长度：Ala22-Gln170

应用：ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

种属：Yersinia pestis

性质：Recombinant

内毒素水平：Please contact with the lab for this information.

纯度：>90% as determined by SDS-PAGE.

状态：Lyophilized

用途范围：仅用于科研

储存：Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

产品介绍：2026年伊尔库茨克抗鼠疫所不明肺炎死亡事件引发肺鼠疫关注，F1重组蛋白在鼠疫抗体检测与疫苗研究中具有重要科研价值。Yersinia pestis F1 capsule antigen/Caf1 Recombinant Protein (NHis)｜JN173012 是艾必赛生物（abinScience）推出的重组鼠疫 F1 荚膜抗原，采用 E. coli 表达 caf1（Accession P26948）成熟段 Ala22Gln170，N 端带 His 标签；经纯化后 SDSPAGE 纯度 >90%，以冻干粉形式提供，冻干前缓冲为 PBS pH 7.4、1 mM EDTA、4% 海藻糖、1% 甘露醇，无菌水重悬后可用于 ELISA、WB、SDSPAGE 及免疫原；生物活性相关验证进行中。短期频繁使用 2–8°C，长期建议－20～－80°C 保存（自收货日起 ≤12 个月），避免反复冻融。

鼠疫耶尔森菌为革兰阴性球杆菌，属肠杆菌科，主要通过染菌跳蚤叮咬、接触感染动物/患者分泌物传播，可致腺鼠疫、肺鼠疫等，肺型可经气溶胶人传人。其 F1（fraction 1）荚膜由 caf1 编码，在 pFra 毒力质粒 caf 操纵子调控下、37°C 宿主体温条件高表达，以伴侣引导（chaperone/usher）方式组装成菌毛样/凝胶样荚膜聚合体；Caf1 可抑制巨噬细胞吞噬、降低上皮细胞摄取并干扰补体替代途径，是菌种相对特异的优势表面抗原。 因此 F1/Caf1 重组蛋白常作为鼠疫抗体筛查、双抗夹心 ELISA/LFIA、抗 F1 抗体效价评估、F1V 亚单位疫苗及免疫原性研究的抗原参照；需注意部分 F1 阴性株仍保持毒力，因此科研中常与LcrV联合评估。

产品购买联系方式：027-87433958/17302791434

邮箱：info@abinscience.com

参考文献：

Optimised Heterologous Expression and Functional Analysis of the Yersinia pestis F1-Capsular Antigen Regulator Caf1R. PMID: 34575967

Fraction 1 capsular antigen (F1) purification from Yersinia pestis CO92 and from an Escherichia coli recombinant strain and efficacy against lethal plague challenge. PMID: 8675324

Characterization of an F1 deletion mutant of Yersinia pestis CO92, pathogenic role of F1 antigen in bubonic and pneumonic plague, and evaluation of sensitivity and specificity of F1 antigen capture-based dipsticks. PMID: 21367990

Rapid detection of Yersinia pestis recombinant fraction 1 capsular antigen. PMID: 25994256

Unraveling the molecular determinants of the anti-phagocytic protein cloak of plague bacteria. PMID: 35358289

Macromolecular organization of the Yersinia pestis capsular F1 antigen: insights from time-of-flight mass spectrometry. PMID: 11604546

[Preparation and biological activity analysis of chimeric antibody against capsular F1 antigen of Yersinia pestis]. PMID: 24321075

Proteomic characterization of Yersinia pestis virulence. PMID: 16291690

Role of fraction 1 antigen of Yersinia pestis in inhibition of phagocytosis. PMID: 11854232

Studies on the contribution of the F1 capsule-associated plasmid pFra to the virulence of Yersinia pestis. PMID: 8833858

Mucosally delivered Salmonella typhi expressing the Yersinia pestis F1 antigen elicits mucosal and systemic immunity early in life and primes the neonatal immune system for a vigorous anamnestic response to parenteral F1 boost. PMID: 19124765

Effective protective immunity to Yersinia pestis infection conferred by DNA vaccine coding for derivatives of the F1 capsular antigen. PMID: 12496187

Complete Protection against Pneumonic and Bubonic Plague after a Single Oral Vaccination. PMID: 26473734

Rapid and sensitive detection of Yersinia pestis by lateral-flow assay in simulated clinical samples. PMID: 30107826

[Influence of different F1-specific components of Yersinia pestis capsular antigen on cell-mediated immunity]. PMID: 16532638

Development of a double-antibody sandwich ELISA for sensitive detection of Yersinia pestis. PMID: 31621104

Development of phage-based single chain Fv antibody reagents for detection of Yersinia pestis. PMID: 22174746

Humoral and cellular immune correlates of protection against bubonic plague by a live Yersinia pseudotuberculosis vaccine. PMID: 30467064

[The genetic control of the F1-specific components of the capsular antigen in Yersinia pestis]. PMID: 10852029

[Chaperone Caf1M stabilizes hybrid proteins containing sequences of F1 antigen subunit from Yersinia pestis]. PMID: 11558261

The Yersinia pestis caf1M1A1 fimbrial capsule operon promotes transmission by flea bite in a mouse model of bubonic plague. PMID: 19103769

[Biological activity of the components of F1-antigen of Yersinia pestis]. PMID: 16830596

Oral vaccination with salmonella simultaneously expressing Yersinia pestis F1 and V antigens protects against bubonic and pneumonic plague. PMID: 17202369

Donor strand complementation mechanism in the biogenesis of non-pilus systems. PMID: 12180918

An encapsulated Yersinia pseudotuberculosis is a highly efficient vaccine against pneumonic plague. PMID: 22348169

[Immunochemical characterization of Fraction I of Yersinia pestis strains by CAF1M gene]. PMID: 11904918

Capsular antigen fraction 1 and Pla modulate the susceptibility of Yersinia pestis to pulmonary antimicrobial peptides such as cathelicidin. PMID: 18227173

Evaluation of a standardized F1 capsular antigen capture ELISA test kit for the rapid diagnosis of plague. PMID: 15145459

Quantitative anti-F1 and anti-V IgG ELISAs as serological correlates of protection against plague in female Swiss Webster mice. PMID: 19925906

Conserved hydrophobic clusters on the surface of the Caf1A usher C-terminal domain are important for F1 antigen assembly. PMID: 20797400

Development and evaluation of a 4-target multiplex real-time polymerase chain reaction assay for the detection and characterization of Yersinia pestis. PMID: 16949784

A novel self-capping mechanism controls aggregation of periplasmic chaperone Caf1M. PMID: 17376079

详情请咨询 abinScience 亚洲区官方子公司 —— 武汉艾必赛生物技术有限公司
官方网址：【中文】https://www.abinscience.cn

【英文】https://www.abinscience.com

武汉艾必赛生物技术有限公司成立于 2023 年，是 abinScience 在亚洲地区设立的官方全资子公司，全面负责 abinScience 品牌在亚太市场的科研试剂推广、销售及技术支持工作。
依托严格的产品研发体系和国际化的质量管理标准，武汉艾必赛与 AtaGenix、ProteoGenix 等知名品牌保持长期战略合作，现已构建起涵盖抗体、重组蛋白、工具酶、试剂盒、实验耗材在内的多品类供应体系，面向区域内科研机构、高校实验室与生物医药企业，提供稳定、高效、可信赖的产品与服务。
核心业务覆盖：
? 抗体产品（单抗、多抗、重组抗体等）
? 蛋白与多肽试剂（标签蛋白、功能蛋白等）
? 分子与细胞实验相关耗材
? 定制化研发服务（蛋白表达、抗体定制、亲和力成熟等）
? 科研供应链整合与项目配套支持
武汉艾必赛始终秉持“Empowering Bioscience Discovery”的理念，为亚太地区的生命科学研究持续注入高质量动能。